RNA-DNA sequence differences in Saccharomyces cerevisiae
- Vivian G. Cheung1,6,
- Isabel X Wang2,
- Christopher Grunseich3,
- Youree G Chang4,
- Girish Ramrattan4,
- Zhengwei Zhang4 and
- Hojoong Kwak5
- 1 HHMI/University of Michigan Life Sciences Institute;
- 2 University of Michigan Life Sciences Institute;
- 3 NIH/NINDS;
- 4 University of Michigan;
- 5 Cornell University
- ↵* Corresponding author; email: vgcheung{at}umich.edu
Abstract
Alterations of RNA sequences and structures, such as those from editing and alternative splicing, result in two or more RNA transcripts from a DNA template. It was thought that in yeast, RNA editing only occurs in tRNAs. Here, we found that Saccharomyces cerevisiae have all 12 types of RNA-DNA sequence differences (RDDs) in the mRNA. We showed these sequence differences are propagated to proteins, as we identified peptides encoded by the RNA sequences in addition to those by the DNA sequences at RDD sites. RDDs are significantly enriched at regions with R-loops. A screen of yeast mutants showed that RDD formation is affected by mutations in genes regulating R-loops. Loss-of-function mutations in ribonuclease H, senataxin and topoisomerase I that resolve RNA-DNA hybrids lead to increases in RDD frequency. Our results demonstrate that RDD is a conserved process that diversifies transcriptomes and proteomes, and provide a mechanistic link between R-loops and RDDs.
- Received April 2, 2016.
- Accepted September 15, 2016.
- Published by Cold Spring Harbor Laboratory Press
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