HCFC1 is a common component of active human CpG-island promoters and coincides with ZNF143, THAP11, YY1 and GABP transcription factor occupancy
- Joelle Michaud1,
- Viviane Praz1,
- Nicole James Faresse1,
- Courtney JnBaptiste1,
- Shweta Tyagi2,
- Frederic Schutz3 and
- Winship Herr1,4
- 1 University of Lausanne;
- 2 Centre for DNA Fingerprinting and Diagnostics;
- 3 Swiss Institute of Bioinformatics
- ↵* Corresponding author; email: winship.herr{at}unil.ch
Abstract
In human transcriptional regulation, DNA-sequence-specific factors can associate with intermediaries that orchestrate interactions with a diverse set of chromatin-modifying enzymes. One such intermediary is HCFC1 (also known as HCF-1). HCFC1, first identified in herpes-simplex virus transcription, has a poorly defined role in cellular transcriptional regulation. We show here that, in HeLa cells, HCFC1 is observed bound to 5400 generally active CpG-island promoters. Examination of the DNA sequences underlying the HCFC1-binding sites revealed three sequence motifs associated with the binding of (i) ZNF143 and THAP11 (also known as Ronin), (ii) GABP, and (iii) YY1 sequence-specific transcription factors. Subsequent analysis revealed co-localisation of HCFC1 with these four transcription factors at approximately 90% of the 5400 HCFC1-bound promoters. These studies suggest that a relatively small number of transcription factors play a major role in HeLa-cell transcriptional regulation in association with HCFC1.
- Received September 28, 2012.
- Accepted March 27, 2013.
- © 2013, Published by Cold Spring Harbor Laboratory Press
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