Targeted gene addition to a predetermined site in the human genome using a ZFN-based nicking enzyme
- Jianbin Wang,
- Geoffrey Friedman,
- Yannick Doyon,
- Nathaniel S. Wang,
- Carrie Jiaxin Li,
- Jeffrey C. Miller,
- Kevin L. Hua,
- Jenny Jiacheng Yan,
- Joshua E Babiarz,
- Philip D. Gregory and
- Michael C. Holmes1
- ↵* Corresponding author; email: mholmes{at}sangamo.com
Abstract
Zinc-finger nucleases (ZFNs) drive highly efficient genome editing by generating a site-specific DNA double-strand break (DSB) at a predetermined site in the genome. Subsequent repair of this break via the non-homologous end-joining (NHEJ) or homology-directed repair (HDR) pathways results in targeted gene disruption or gene addition, respectively. Here we report that ZFNs can be engineered to induce a site-specific DNA single-strand break (SSB) or nick. Using the CCR5-specific ZFNs as a model system we show that introduction of a nick at this target site stimulates gene addition using a homologous donor template, but fails to induce significant levels of the small insertions and deletions (indels) characteristic of repair via NHEJ. Gene addition by these CCR5-targeted zinc finger nickases (ZFNickases) occurs in both transformed and primary human cells at efficiencies of up to ~1-8%. Interestingly, ZFNickases targeting the AAVS1"safe harbor" locus reveal similar in vitro nicking activity, a marked reduction of indels characteristic of NHEJ but stimulated far lower levels of gene addition - suggesting that other, yet to be identified, mediators of nick induced gene targeting exist. Introduction of a site-specific nicks at distinct endogenous loci provide an important tool for the study of DNA repair. Moreover, the potential for a SSB to direct repair pathway choice (i.e. HDR but not NHEJ) may prove advantageous for certain therapeutic applications such as the targeted correction of human disease-causing mutations.
- Received March 2, 2011.
- Accepted March 7, 2012.
- Copyright © 2012, Cold Spring Harbor Laboratory Press
