Regulation of nucleosome landscape and transcription factor targeting at tissue-specific enhancers by BRG1
- Gangqing Hu1,
- Dustin Schones2,
- Kairong Cui2,
- River Ybarra3,
- Daniel Northrup2,
- Qingsong Tang2,
- Luca Gattinoni4,
- Nicholas Restifo4,
- Suming Huang3 and
- Keji Zhao5,6
- 1 National Heart, Lung and Blood Institute;
- 2 Laboratory of Molecular Immunology, National Heart, Lung and Blood Institute, NIH;
- 3 Department of Biochemistry and Molecular Biology, University of Florida College of Medicine;
- 4 Center for Cancer Research, National Cancer Institute, NIH;
- 5 NHLBI-NIH
- ↵* Corresponding author; email: zhaok{at}nhlbi.nih.gov
Abstract
Enhancers of transcription activate transcription via binding of sequence-specific transcription factors to their target sites in chromatin. In this report, we identify GATA1-bound distal sites genome-wide and find a global reorganization of the nucleosomes at these potential enhancers during differentiation of hematopoietic stem cells (HSCs) to erythrocytes. We show that the catalytic subunit BRG1 of BAF complexes localizes to these distal sites during differentiation and generates a longer nucleosome linker region surrounding the GATA1 sites by shifting the flanking nucleosomes away. Intriguingly, we find that the nucleosome shifting specifically facilitates binding of TAL1 but not GATA1, and are linked to subsequent transcriptional regulation of target genes.
- Received January 20, 2011.
- Accepted June 21, 2011.
- Copyright © 2011, Cold Spring Harbor Laboratory Press
