Allele-specific methylation is prevalent and is contributed by CpG-SNPs in the human genome
- * Corresponding author; email: k4zhang{at}ucsd.edu
Abstract
In diploid mammalian genomes, parental alleles can exhibit different methylation patterns (allele-specific DNA methylation, ASM), which have been documented in a small number of cases except for the imprinted regions and X chromosomes in females. We carried out a chromosome-wide survey of ASM across 16 human pluripotent and adult cell lines using Illumina bisulfite sequencing. We applied the principle of linkage disequilibrium (LD) analysis to characterize the correlation of methylation between adjacent CpG sites on single DNA molecules, and also investigated the correlation between CpG methylation and single nucleotide polymorphisms (SNPs). We observed ASM on 23~37% heterozygous SNPs in any given cell line. ASM is often cell-type specific. Furthermore, we found that a significant fraction (38~88%) of ASM regions are dependent on the presence of heterozygous SNPs in CpG dinucleotides that disrupt their methylation potential. This study identified distinct types of ASM across many cell types and suggests a potential role for CpG-SNP in connecting genetic variation with the epigenome.
Footnotes
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- Received December 29, 2009.
- Accepted April 15, 2010.
- Copyright © 2010, Cold Spring Harbor Laboratory Press
