Characterization of the RNA content of chromatin
- * Corresponding author; email: kanduri.chandrasekhar{at}genpat.uu.se
Abstract
Noncoding RNA (ncRNA) constitutes a significant portion of the mammalian transcriptome. Emerging evidence suggests that it regulates gene expression in cis or trans by modulating the chromatin structure. To uncover the functional role of ncRNA in chromatin organization, we deep sequenced chromatin associated RNAs (CARs) from human fibroblast (HF) cells. This resulted in the identification of 141 intronic regions and 74 intergenic regions harbouring CARs. The intronic and intergenic CARs show significant conservation across 44 species of placental mammals. Functional characterization of one of the intergenic CARs Intergenic10 revealed that it regulates gene expression of neighbouring genes through modulating the chromatin structure in cis. Our data suggests that ncRNA is an integral component of chromatin and that it may regulate various biological functions through fine-tuning of the chromatin architecture.
Footnotes
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- Received November 23, 2009.
- Accepted April 12, 2010.
This manuscript is Open Access.
- Copyright © 2010, Cold Spring Harbor Laboratory Press
