The completion of the Mammalian Gene Collection (MGC)
Abstract
Since its start, the Mammalian Gene Collection (MGC) has sought to provide at least one full-CDS cDNA clone for every human and mouse gene with a RefSeq transcript, and at least 6200 rat genes. The MGC cloning effort initially relied on random EST-screening of cDNA libraries. Here we summarize our recent progress using directed RT-PCR cloning and DNA synthesis. The MGC now contains clones with the entire protein-coding sequence for 92% of human and 89% of mouse genes with curated RefSeq (NM-accession) transcripts, and for 97 % of human and 96% of mouse genes with curated RefSeq transcripts that have one or more PubMed publications, in addition to clones for more than 6300 rat genes. These high-quality MGC clones and their sequences are accessible without restriction to researchers worldwide.
Footnotes
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- Received May 12, 2009.
- Accepted September 8, 2009.
