Integrated physical map of the 12q22 deleted region in male GCTs. The map between the interval SHGC-33859 (proximal) and D12S1100 (distal) is composed of 42 previously mapped YACs and 168 BACs, 49 PACs, and 52 cosmids isolated in this study. The solid green bar (top) represents the chromosomal region in proximal to distal orientation. The markers ordered on the contig are shown above the green bar (distances in the map are not to scale). All markers shown in green and P382A8/AG (shown in black) were generated in this study by sequencing ends of the clones. Markers are color-coded as follows: (blue and green) nonpolymorphic; (red) EST/gene; (black) polymorphic. Red brackets above the markers indicate that the ESTs are in the same unigene cluster. Small black brackets above the green line indicate that the relative order of the markers could not be determined unambiguously. The numbers below the green line indicate the genetic map position on chromosome 12 (Généthon map). The first level of the map below the green line represents YACs (black lines); the second level (below the YAC contig) represents BAC, PAC, and cosmid clone contig. The prefix B denotes BACs (black), P denotes PACs (green), and c denotes cosmids (blue). The STS content of each clone is indicated by symbols: (black circle) polymorphic marker; (blue square) nonpolymorphic marker; (blue square with red border) clone-end marker generated by sequencing; (downward red triangle) EST; (upward red triangle) gene; (upward red triangle with blue border) gene sequence identified from an end sequence. Open symbols indicate that the marker was not tested. A bracket within YAC or BAC clone indicates lack of marker(s), which may represent internal deletion. The large bidirectional arrow (top) indicates the minimal region of deletion. BAC clones drawn in thick, black lines represent minimal tiling path. Underlined clones indicate fingerprinted clones.
