Table 2.
Sequence and Position of PCR Primers
| Primer | Position | Sequence (5′ to 3′) |
| UNI-33 | TTTTTTTTTTTTTTTGTTTGTTGTGGGGGGGTT | |
| UNI-17 | TTTTTGTTTGTTGTGGG | |
| Op1− | +94 | TGCTGGACTGGGTGCTGTCCTCATAGC |
| Op2− | +78 | TCATAGCTGTCCTGCGGATGGCG |
| Op1+ | +254 | CCAGTAGCTGGGATTACAGG |
| Op2+ | −151 | TGGGAGGAGGAGGTCTAAGTCC |
| Up(A−) | −130 | GCTCCCAAATCCTACACCATC |
| Up(B−) | −233 | AGAGGGATTTGCTGGAAAC |
| Up(C−) | −2350 | AGACAGTAGACAGGTGGGTGCC |
| MW− | −114 | AAATCCTACACCATCTGATCTC |
| MW+ | −865 | AGGTGATCCGGGAATAGTAAG |
| NWMLCR− | −360 | CTGTCCAGTGCCCGCCTTTGG |
| MarUp(A+) | −627 | GAAGGAGCTGCAGCTCATGG |
| MarLCR(C+) | −2896 | CCTCAGAATTGCTTATCTCCCTC |
| MarLCR(D−) | −1015 | TGCCTCCTTTTGGGTTTGTTCC |
| MarLCR(D+) | −2221 | TTTGTTTATTCAATCATCCG |
| MarLCR(E−) | −1257 | GTGCTAGGTGCCAAGCTAGG |
| MarLCR(E+) | −1900 | CGATGGACACAGTTTGCTTCC |
| LCR(C+) | −3484 | GACTTGATCTTCTGTTAGCCCTAATC |
| AL− | −380 | GGATGATGAATCTGCCCAGAG |
| AL+ | −3225 | CGAAGGTGCAGAACCTACCG |
-
Except for Op1+, Op1− and Op2−, which are based on the human MW and LW sequences, and AL−, which is based on the howler monkey sequence, the nucleotide numbering system refers to the marmoset X-linked opsin gene, where +1 would be the first base of the coding sequence.
