MnCit addition improves detection of heterozygous mutations. Compared are DNA sequence electropherograms produced by sequencing different pairs of plasmids with dRhodamine reagents in the absence (−) and the presence (+) of 0.2 mm (A) or 0.3 mm(B,C) MnCit. The templates were approximately equimolar mixtures of two plasmids containing segments of DNA that differed by single nucleotides. The arrows and letters indicate the sites of a suppressed G (G sup); mixed peaks are attributable to the DNA sequences corresponding to heterozygous mutations (e.g., G + A), with the upper letter being the taller peak; and a T peak (T) that is strong in the template with the sequence 5′–GATC–3′, but not in the template with the sequence 5′–AATC–3′ (data not shown).
