Increasing MnCit concentrations decrease the CVs of peak heights of different PE/ABI dye terminator sequencing kits. The data were obtained by sequencing with the kits, templates, and primers indicated. The curves of the CV values using the −21/pUC, M13F, M13R primers were determined by measuring the height of 100 nucleotide peaks as described in Methods, except the BigDye kit results with the −21/pUC primer are based on the fifty nucleotides 55–104. The dRhodamine and the BigDye sequencing results that contain the indicated amounts of EDTA were produced using the control −21/pUC primer and DNA template from the kits; whereas, the other results were obtained using our preparations of primers and pGEM3Zf(+) DNA that were dissolved in water.
