Table 2.
Effect of Eliminating Replicate Ditags on Tag Counts
| Tag (CATG + . . .) | Identity | Frequency including replicate ditags | Frequency excluding replicate ditags | Decrease after excluding replicates (%) |
| CCCATCGTCC | COX2 | 1348 | 890 | 34 |
| AAGATCAAGA | actin | 756 | 523 | 31 |
| TGATTTCACT | COX3 | 316 | 240 | 24 |
| GGGGAGGAAC | slow troponin T | 205 | 169 | 18 |
| CAAGTATAAA | titin | 134 | 117 | 13 |
| TTTACTCAGC | C protein | 58 | 55 | 5 |
| CCTCTCTGGT | EST F20783 | 34 | 34 | 0 |
| TCTTGTGCAT | LDH A | 10 | 10 | 0 |
| AACAGCTCCC | EST F19683 | 6 | 6 | 0 |
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These tag counts do not match those in Table 1 because they represent only half of the data. Because the underestimation of the most abundant tags would have become progressively greater with an increasing number of total tags, two separate databases were created and merged to produce the data in Table 1. Thus, the underestimation for the combined databases does not exceed the percentages shown here. (COX2) Cytochrome c oxidase 2; (COX3) cytochrome c oxidase 3; (LDH A) lactate dehydrogenase A.
