Inventory of High-Abundance mRNAs in Skeletal Muscle of Normal Men

Table 1.

Skeletal Muscle SAGE Tags Detected 100 or More Times in a Sample of 53,875 Total Tags

Tag (CATG + . . .) Gene product GenBank accession no. Times detected
CCCATCGTCC cytochrome c oxidase 2 7627–40 1780
AAGATCAAGA α actin J00068 1001
ATCCCCGCCC creatine kinase M M14780 851
TTCATACACC NADH dehydrogenase 4/4L 11491-504 847
CTGGAGCCTG type 1 (slow, cardiac β) myosin heavy chain X05631 830
CCCACCACCC β-tropomyosin X06825 703
AGCCCTACAA NADH dehydrogenase 3 9709–22 702
TACCATCAAT glyceraldehyde-3-phosphate dehydrogenase M36164 593
GCGACCGTCA fructose 1,6-diphosphate aldolase a M21190 582
GTTTGGATCT myoglobin X00373 581
ACCCTTGGCC NADH dehydrogenase 1 3263–76 565
GAATGACTGA myosin heavy chain 2a Z32858 563
TCCTCAACCC slow twitch troponin C M37984 499
TGATTTCACT cytochrome c oxidase 3 8726–39 489
ACTAACACCC NADH dehydrogenase 2 4606–19 478
CTAAGACTTC 16 S rRNA 2276–89 442
TGGGCGGCCT myosin light chain 2 M21812 409
CCCCGGCCAC desmin U59167 400
GAGGGCCGGA fast troponin I L21715 400
ATGGTGCGCC fast skeletal troponin C X07898 397
AGGCACCTGG EST similar to slow twitch skeletal muscle troponin I N87243 F17753 393
TTCCAATAAA cardiac myosin light chain 2 X66141 388
GGGGAGGAAC slow troponin T M19309 364
AAAACATTCT truncated 16 S rRNA 1738–51 325
AGGATCGAGG β enolase X51957 324
CACCTAATTG ATPase 6/8 8460–73 304
GGAGCCAACT EST from muscle, similar to troponin T AA192217 287
CAGAGGGTGG glycogen phosphorylase X03031 285
TAGGTTGTCT translationally controlled tumor protein L13806 277
AATCAACAAA myosin light chain 3f X05451 257
CAAGCATCCC truncated 12 S rRNA 118–31 248
CACTACTCAC cytochrome b 14326–39 238
CAAGTATAAA titin X69490 208
CCTCAGGATA NADH dehydrogenase 5 13853–66 186
GAGGCTGTGG phosphoglycerate mutase muscle specific subunit M18172 186
ATTTGAGAAG cytochrome c oxidase 1 6737–50 177
GGGCTGGGGT ribosomal protein L29 U10248 175
GCTTTGCCTC cytochrome c oxidase 6a M83308 169
TCGAAGCCCC NADH dehydrogense 4L 10841–54 169
AAGACAGTGG ribosomal protein L37a X66699 163
TGGGCAAAGC elongation factor 1γ Z11531 163
AAAGTCATTG α-tropomyosin M19713 146
TGCCAGAAAA telethonin AJ000491 142
AGCACCTCCA elongation factor 2 Z11692 131
TTGGTCCTCT ribosomal protein L41 AF026844 131
TAATGACAAT skeletal muscle LIM-protein FHL1 U60115 128
TGAATAAAGT sarcolipin U96093 124
TTCAATAAAA acidic ribosomal phosphoprotein P1 M17886 117
TTTACTCAGC skeletal muscle C-protein (slow myosin binding protein C) X66276 111
X73114
CTCATAGCAG EST similar to translationally controlled tumor protein AA716547 110
TTGGAGATCT NADH dehydrogenase MLRQ subunit U94586 110
GTTTCAGGTA Ca2+ ATPase (HK2) M23115 107
AGGGCTTCCA Wilm’s tumor-related protein QM M642421 103
TCTGCACCTC S1 (elongation factor-1 α2) X70940 100
  • Complete lists of all tags, in both alphabetical order and order of abundance, are available via the internet athttp://www.gcrc.rochester.edu/SWindex.html.

  • Italics denotes tags matching the mitochondrial genome (HSMITG, GenBank accession no. X93334), and values listed under accession number for these tags indicate the locus within the mitochondrial genome.

  • Also matches other actin isoforms, but α-actin is much more abundant in skeletal muscle.

  • Results from present study, Lanfranchi et al. (1996), andWade et al. (1989) have A in eighth position; X05451 has G in eighth position.

This Article

  1. Genome Res. 9: 506-513

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