Map of the NlaIII restriction sites in the mitochondrial genome (HSMITG, GenBank Accession no. X93334), and abundances of the SAGE tags corresponding to each of these sites in a sample of 53,875 total tags (excluding replicate ditags). Mitochondrial DNA is circular, but is shown here as several linear pieces to facilitate presentation. Numbers at end of each segment indicate base number in HSMITG, which corresponds to the base sequence of the RNA encoded by the heavy strand (H strand). Numbers under each arrow indicate the frequency of tags corresponding to the H strand transcripts, which include both rRNAs and 12 of the 13 mRNAs (only NADH6 mRNA is encoded by L strand). Numbers above arrows indicate frequency of L strand transcripts corresponding to each site (if no number is shown, no tags matching that site were detected). The presence of a tag indicates that the transcript was polyadenylated somewhere between that NlaIII site and the next one, or between that NlaIII site and the next tRNA (tRNAs are spliced from primary transcript but not polyadenylated). The tRNA genes and control regions are shaded. Single-letter amino acid abbreviations identify location of specific tRNAs (those encoded by H strand indicated below the shaded area, those encoded by the L strand indicated above the shaded area). TheNADH1 tag occurring 21 times (asterisk) matches HUMMTCG (GenBank accession no. J01415) rather than HSMITG (G rather than A at base 2740 of HSMITG). [NADH(n)]NADH dehydrogenase(subunit); [COX(n)]cytochrome c oxidase (subunit); (CYT b) cytochrome b.
