Fluorescence Polarization in Homogeneous Nucleic Acid Analysis

Table 3.

FP–TDI Assay with Synthetic Templates Using Different Dye Terminators

Templates BFL–ddA (mP) TMR–ddC (mP) ROX–ddG (mP) BTR–ddU (mP)
CF508–48A 38 43 77 174
37 53 73 175
31 36 78 174
35 49 82 170
CF508–48C 20 50 214 32
19 37 209 27
20 56 215 25
14 38 207 26
CF508–48G 23 247 84 23
24 266 80 30
22 253 75 23
15 262 74 21
CF508–48T 113 52 81 32
106 41 68 39
108 59 81 30
103 32 76 28
Avg. ctrl. 25 46 86 28
s.d.Ctrl. 8.2 8.8 4.6 5.0
Avg. net chg. 83 211 134 145

  • Positive reactions are in boldface type.

  • ↵FP measurements for BFL were made with excitation at 480 nm and emission at 520 nm; for TMR the excitation was 535 nm and emission was 590 nm; for BTR the excitation was 591 nm and emission was 635 nm; for ROX the excitation was 580 nm and emission was 620 nm.

  • ↵Net change over average of control.

This Article

  1. Genome Res. 9: 492-498

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