Fluorescence Polarization in Homogeneous Nucleic Acid Analysis

Table 2.

FP–TDI Assay with Synthetic Templates Using FAM-Labeled Dye Terminators

Templates	FAM–ddA (mP)	FAM–ddC (mP)	FAM–ddG (mP)	FAM–ddU (mP)
CF508–48A	52	36	54	89
	55	37	41	92
	52	39	48	101
	50	39	40	93
CF508–48C	57	37	121	39
	50	37	126	30
	55	39	115	40
	52	34	117	40
CF508–48G	52	92	42	42
	63	85	35	32
	50	91	40	47
	49	103	37	35
CF508–48T	186	32	48	34
	180	38	63	41
	183	36	43	33
	179	33	55	45
Avg. ctrl.	53	36	46	38
s.d.Ctrl.	4.0	2.5	8.3	5.3
Avg. net chg.	129	57	74	55

Positive reactions are in boldface type.
↵FP measurements for FAM were made with excitation at 485 nm and monitored at 530 nm.
↵Net change over average of control.

This Article

Genome Res. 1999. 9: 492-498

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