Loss of Heterozygosity Analysis Using Whole Genome Amplification, Cell Sorting, and Fluorescence-Based PCR

Table 1.

Comparison Between Normal and Aneuploid Allele Ratios

Ploidy (N) Aneuploid 9p 9q
No. % D9S925 D9S1121 D9S1118 D9S301 D9S930
NL 1.14 N.I. 1.65 1.01 1.08
3.0 1 34.5 An 0.04 N.I. 0.00 0.02 0.02
NL N.I. 1.15 N.I. 1.15 0.86
3.5 2 30.9 An N.I. 0.12 N.I. N.D. 0.19
NL 1.36 1.47 1.19 1.13 1.23
3.5 3 32.4 An 0.00 0.00 0.00 1.23 1.07
NL 0.98 N.I. N.D. 1.29 1.38
1.8 4 74.6 An 0.00 N.I. N.D. 1.07 0.97
NL 0.76 N.I. N.D. N.D. 1.04
4.0 5 62.6 An 0.00 N.I. 0.00 1.17 1.37
NL 1.31 1.02 0.96 1.11 1.01
3.2 6 60.1 An 0.01 0.05 0.01 0.44 0.60
NL 1.02 1.35 1.15 N.I. 1.06
2.9 7 77.7 An 0.00 0.01 0.00 N.I. 0.55
NL 1.00 1.27 1.48 1.14 1.31
3.4 8 34.40 An 0.00 0.03 0.00 0.60 0.81
NL 1.07 1.11 1.20 1.10 1.03
3.3 9 58.7 An 0.00 0.00 0.00 1.07 0.01
NL 1.05 N.I. N.I. 0.90 1.15
3.5 10 12.9 An 0.16 N.I. N.I. 1.04 N.D.
  • Average allele ratios for four individual PEP reactions for normal control (NL) and flow-purified aneuploid (An) populations from 10 patients with esophageal adenocarcinoma. Ploidy and percent aneuploid were measured by flow cytometry. (N.I.) Not informative; (N.D.) no data.

This Article

  1. Genome Res. 9: 482-491

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