Genetic Localization of a Drosophila melanogaster Resistance Gene to a Parasitoid Wasp and Physical Mapping of the Region

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Figure 2.
Figure 2.

Localization of P1 and cosmid clones in a 55C–56A contig: Thirty-one cosmid and thirty-three P1 clones supposed to be located in55C–56A or not localized yet, were analyzed by cross-hybridization experiments to obtain a contig of this region. Four contigs (2, 4, 11, and 5 clones) were obtained with the cosmids and three contigs with the P1s (4, 4, and 10 clones). Cosmids are represented by thick lines and P1s by empty boxes. Both P1 and cosmid libraries proved necessary to determine a complete contig. Vertical arrows indicate in situ localization of three of the clones, 119c2 (55C1–2/C3/C4–5), 27B7 (55D1–2/D3), and 59G5 (55 F6–F13) which allowed to demonstrate that the entire55C–55F region had been recovered. Finally, an estimated localization of the 55E2–6; 55F3 region, based on BDGP in situ data is given.

This Article

  1. Genome Res. 9: 471-481

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