An Automated Sample Preparation System for Large-Scale DNA Sequencing
Abstract
Recent advances in DNA sequencing technologies, both in the form of high lane-density gels and automated capillary systems, will lead to an increased requirement for sample preparation systems that operate at low cost and high throughput. As part of the development of a fully automated sequencing system, we have developed an automated subsystem capable of producing 10,000 sequence-ready ssDNA templates per day from libraries of M13 plaques at a cost of $0.29 per sample. This Front End has been in high throughput operation since June, 1997 and has produced > 400,000 high-quality DNA templates.
Footnotes
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↵1 Corresponding author. Present address: Department of Physics and Astronomy, University of British Columbia, Vancouver, British Columbia V6T 1Z1 Canada.
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E-MAIL andre{at}physics.ubc.ca; FAX (604) 822-5324.
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- Received November 18, 1998.
- Accepted March 24, 1999.
- Cold Spring Harbor Laboratory Press
