Table 2.
Summary of Cloning and Expression Results in pcDNA3.1/GS for Yeast ORFs and Human Kinases
| Process | Success rate of yeast ORFs (%) | Success rate human kinases (%) | |||
| first pass | second pass | ||||
| RT–PCR/human kinases | N.A. | N.A. | 62 | ||
| ORF amplification/yeast | 93 | 93 | N.A. | ||
| Topoisomerase I-mediated cloning + orientation | 67 | 64 | 78 | ||
| Expression QC positive | 15 | 37 | 77 | ||
| Start-to-finish summary | 9 | 22 | 38 | ||
-
(N.A.) Not applicable.
