WWW link to confirming alignments. (A) Alignments of the mouse class-mu EST sequence gi‖2403980 with the original query protein sequences. Two alignments (gtxa_tobac andgtxa_arath) with the original query sequences used to build the histogram in Fig. 5 are shown; alignments tosspa_ecoli [E() < 10−5] andgtx1_soltu [E() < 10−10] are not shown. Alignments were calculated with tfasty3 using BLOSUM50 and gap penalties of −15/−3/−20/−20 (Pearson et al. 1997). (−) Protein or translated DNA alignment gaps; (\,/) frameshifts. (Dashed Line) A map of the portion of the query amino acid sequence aligned with the EST sequence. (B) A re-search of thegi‖2403980 EST against the SwissProt database. Significant similarity is shared with several plant glutathione transferases. Alignments are shown with a C. elegansunidentified glutathione transferase and a hypotheticalNicotiana enzyme.
