Figure 2.
Glutathione transferase evolutionary tree. One-hundred thirty-six glutathione transferase family members were identified by searches of SwissProt (rel. 34 with updates) with the query sequences GTP_HUMAN, GTT1_DROME, GTS2_DROME, GTH1_TOBAC, and GT_HAEIN. The protein sequences were aligned using ClustalW (Thompson et al. 1994), and a distance matrix was calculated with the PHYLIP protdist program (Felsenstein 1989). The tree shown was produced by thefitch program of the PHYLIP package. The regions of the tree used for gene discovery are highlighted in color.
