A Contiguous 3-Mb Sequence-Ready Map in the S3–MX Region on 21q22.2 Based on High- Throughput Nonisotopic Library Screenings

Clone sizes (kb) indicated in brackets were generally estimated by PFGE, or by the mean FPC value form EcoRI andHindIII patterns⁽¹⁾ by the FPC value fromEcoRI pattern⁽²⁾ or from genomic sequencing⁽³⁾. Overlaps (in kb) between clones estimated by FPC after EcoRI or HindIII digestion are shown with the corresponding probability value: (nso) no significant overlap; (/) no FPC data available. When available, overlaps calculated from genomic sequence are given (in kb). Clone to clone overlaps were also calculated in percent of the actual length spanned by a given pair of clones. Cosmids are prefixed with a Q and BACs with a B. The sequenced clones are indicated with an x in the first column. Sequences are available at ^a http://genome.imb-jena.de (Note: cosmids are displayed in 384-well format on this server; Q73F12 = 19K23, Q41G8 = M1511, Q7G4 = 2N7, Q82G10 = 21M20, and Q78B7-20C22) or at^b http://seq.mpimg-berlin-dahlem.mpg.de (identical names).