A Cluster of ABA-Regulated Genes on Arabidopsis thaliana BAC T07M07

  1. Ming Li Wang1,2,3,
  2. Stephen Belmonte2,
  3. Ulandt Kim2,4,
  4. Maureen Dolan2,5,
  5. John W. Morris2,6, and
  6. Howard M. Goodman1,2,7
  1. 1Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 USA; 2Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114 USA

Abstract

Arabidopsis thaliana BAC T07M07 encoding the abscisic acid-insensitive 4 (ABI4) locus has been sequenced completely. It contains a 95,713-bp insert and 24 predicted genes. Most putative genes were confirmed by gel-based RNA profiling and a cluster of ABA-regulated genes was identified. One of the 24 genes, designatedPP2C5, encodes a putative protein phosphatase 2C. The encoded protein was expressed in Escherichia coli, and its enzyme activity in vitro was confirmed.

[The sequence data described in this paper have been submitted to GenBank under accession no. AF085279.]

Footnotes

  • Present addresses: 3Cereon Genomics, LLC., Cambridge, Massachusetts 02139 USA; 4Marine Biological Laboratory, Woods Hole, Massachusetts 02543 USA; 5DuPont Agricultural Products, Newark, Delaware 19711 USA; 6Millennium Pharmaceuticals Inc., Cambridge, Massachusetts 02139 USA.

  • ↵7 Corresponding author.

  • E-MAIL Howard.Goodman{at}molbio.mgh.harvard.edu; FAX (617) 726-3535.

    • Received November 5, 1998.
    • Accepted January 29, 1999.
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  1. doi: 10.1101/gr.9.4.325 Genome Res. 9: 325-333 Cold Spring Harbor Laboratory Press

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