An Efficient DNA Sequencing Strategy Based on the Bacteriophage Mu in Vitro DNA Transposition Reaction

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Figure 3.
Figure 3.

Distribution of 46 Mu transposon insertions in the 5727-bpKpnI fragment. Insertions into this fragment were screened by restriction analysis and appropriate clones were randomly chosen for sequence analysis. The cumulative number of insertions is plotted against the coordinates in the final sequence. (Top) 1-bp resolution; (bottom) 500-bp resolution. On the basis of this analysis, the distribution appears to be relatively even; totally even distribution would produce a line as shown. The result implies that, at least in the scale relevant for DNA sequencing, the target site selection can be described as a random or near-random process.

This Article

  1. Genome Res. 9: 308-315

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