Analysis of Sequence-Tagged-Connector Strategies for DNA Sequencing

Table 2.

Results for a 3-Gb Target with Repeat Families, with Decision Rulek Chosen to Minimize the Total Cost in Each Case, Using Clones of Length C = 150 kb

Coverage S Decision rule k Number of gaps Bases missing Problem clones Clones to sequence Total cost (Gb) Total cost ($millions) Cost of STC resource (%)
10 369 9.08 136,200 24.8 22,336 25.343 $760.30 0.6
15 370 0.09 918 9.5 21,539 24.492 734.77 1.0
20 370 0.00 6 12.5 21,147 24.138 724.15 1.3
25 371 0.00 0 7.1 20,935 23.967 719.02 1.7
30 371 0.00 0 8.5 20,782 23.879 716.38 2.0
35 371 0.00 0 9.9 20,675 23.842 715.25 2.3
38.83 371 0.00 0 11.0 20,612 23.834 715.03 2.6
40 371 0.00 0 11.3 20,595 23.835 715.05 2.7
45 372 0.00 0 5.6 20,546 23.847 715.40 3.0
50 372 0.00 0 6.2 20,496 23.872 716.15 3.4
  • See Fig. 3.

  • Computed for a cost unit of $0.03 (see text).

  • Minimum total cost, over all S.

  • As percent of total cost.

This Article

  1. Genome Res. 9: 297-307

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