Detecting Selective Expression of Genes and Proteins

Table 4.

Examples: The Selective Expression Algorithm Applied to Synthetic, yet Realistic, Data

Example Baseline adjusted λ gap τ log10(sp) d Comments
1a no 0.55 0.67 −6.26 0.33 reference example
1b yes 1.00 0.55 0.67 −6.27 0.33 same as 1a; λ has no effect
2a no 0.28 0.68 −6.26 0.24 d different from 1a due to  gap only
2b yes 0.99 0.28 0.68 −6.28 0.24 d different from 1a due to  gap; λ has no effect
3a no 0.28 0.68 −6.26 0.24 d different from 1a due to  gap only
3b yes 0.87 0.28 0.58 −4.90 0.00 d different from 1a due to  λ−adjusted log10(sp) > −5,  hence d = 0
  • The example identification (1, 2, or 3) corresponds to Fig. 9; whether a baseline compression adjustment was omitted (a) or used (b) in the discordancy computation (equation 6 or 11). The intensities and source quality weights are from Table 3.

  • Equation .

  • Equation .

  • Equation .

  • Equation or 11.

  • Equation . Equation sigmoidal parameters areb = 10 and c = 0.8. The parameter values in the decision function d (equations 13–15) are α = β = γ = 1.5, δ = 0.3, g thresh = 0.25, log10(sp)thresh = −5, and log10(sp) = −20.

  • λ has no effect, as the baseline (i.e., ∼0.3, Table 3) is distant from the maximum allowed intensity (i.e., 1).

  • λ has non-negligible effect, as the baseline is near (i.e., 0.67, Table 3) the maximum intensity.

This Article

  1. Genome Res. 9: 282-296

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