Postsynaptic α-Neurotoxin Gene of the Spitting Cobra, Naja naja sputatrix: Structure, Organization, and Phylogenetic Analysis

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Figure 1.Figure 1.
Figure 1.

Strategies used for PCR and genome walking. (A) Locations of primers used to amplify the NTX gene by PCR and comparison of the gene organization of NTX (NTX; N. n. sputatrix), cobrotoxins (Cbt and Cbt b; N. n. atra) and erabutoxin c (Ec; L. semifasciata). (Solid and open boxes) Exons and introns, respectively. The amino acids corresponding to each exon are also indicated. (B) Agarose gel electrophoresis of PCR products from upstream (lane 1) and downstream (lane 4) of theNTX gene generated by genome walking. (Lanes 2,3) Genomic fragments of NTX gene containing the coding region for signal peptide and matured toxin respectively. (Lane5) Amplification of the complete NTX gene(s) fromN. n. sputatrix.

This Article

  1. Genome Res. 9: 259-266

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