Identified F. rubripes Genes on a Stretch of 148 kb of Genomic DNA
| A: Genes with vertebrate homologs | ||||
| Fugu and human name | Length of Fugu protein (amino acids) | Percent identity | Accession no. | Assigned to chromosome |
| ACVR1B | 506 | 83 human | P36896 | 12q13 |
| 88 zebrafish | X94119 | |||
| ALR | 4825 | 45 human | 2358285 | 12q13 |
| wnt1 | 370 | 76 human | P04628 | 12q13 |
| 92 zebrafish | P24257 | |||
| wnt10b | 390 | 60 human | 1932789 | 12q13 |
| 60 mouse | P48614 | |||
| ARF3 | 181 | 100 human | P16587 | 12q13 |
| erbB3 | 1328 | 51 human | P21860 | 12q13 |
| PAS1 | 392 | 79 human | 1216526 | 12q13 |
| 79 mouse | P50580 | |||
| L41 | 25 | 100 human | P28751 | N.A. |
| LRP1 | 1581 | 72 human | Q07954 | 12q13 |
| (partial) | 74 chicken | P98157 | ||
| B: New genes of a known gene family | ||||
| Fugu name | Length of Fugu protein (amino acids) | Closest homolog | Accession no. | Percent identity (amino acids) |
| Ikaros-like | 417 | Ikaros/Lyf-1 (human) | 1289371 | 85 (zinc finger domains) |
| 50 (full protein) | ||||
| fugu hedgehog (fhh) | 442 | shh (human) | 663157 | 48 |
| shh (mouse) | A49425 | 52 | ||
| ihh (mouse) | 1890097 | 52 | ||
| C. New vertebrate genes | |||||
| Fugu name | Length of Fugu protein (amino acids) | No. of exons | Significant hits with dbEST entries (acc. no.) | Exons | E-value |
| R05D3.2-like | 497 | 15 | AA307511 | 3–8 | 8e-58 |
| AA037301 | 12–15 | 1e-39 | |||
| AA304712 | 11–14 | 1e-27 | |||
| AA307337 | 8–13 | 4e-23 | |||
| 138e3.2 | 255 | 4 | HUMHBC4912 | 2 | 3e-15 |
| AA905621 | 4 | 2e-06 | |||
| AA170127 | 3–4 | 4e-11 | |||
| 178o23.1 | 966 | 23 | T27154 | 3–8 | 6e-54 |
| H82462 | 4–9 | 6e-35 | |||
| AA312185 | 15–17 | 4e-29 | |||
| AA378749 | 9–11 | 6e-18 | |||
| AA773555 | 21–23 | 5e-16 | |||
| H10578 | 13–14 | 7e-13 | |||
| R88176 | 1 | 4e-09 | |||
| diaphanous-like | 1037 | 24 | AA309980 | 17–19 | 4e-48 |
| AA224424 | 19–22 | 3e-46 | |||
| T07474 | 9–12 | 4e-39 | |||
| AA459557 | 5–7, 9–10 | 4e-39 | |||
| U69179 | 17–23 | 9e-32 | |||
| Z41620 | 15–16 | 9e-28 | |||
| AA421752 | 4–5 | 1e-14 | |||
| M78344 | 23–24 | 1e-09 | |||
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Homology searches were made with the putative amino acid sequences of the identified Fugu genes using the GAP-BLAST program (Altschul et al. 1997). (A) Fugu genes with highly homologous human counterparts. Genomic location data for human and mouse were obtained from NCBI; http://www.ncbi.nlm.nih.gov. (N.A.) Data not available. (B) New Fugu genes belonging to a known vertebrate gene family. (C) Fugu genes with no or only weak homology to any characterized vertebrate gene. Strong homologies to human entries of the dbEST library can be found for all four Fugu genes.
