Mining SNPs From EST Databases

Summary of GBA results. Forty-four candidate EST–SNP sites were confirmed by GBA analysis; a subset of six sites (*) were also confirmed by fluorescent-based sequencing. Multiple SNPs were assayed in contig 14. The sequence context surrounding the base substitution (major allele/minor) is noted. The allele frequency data per ethnic group, and on average, were calculated based on GBA data from a total of 36 chromosomes: 8 individuals (16 chromosomes) for the Caucasian population and 5 individuals (10 chromosomes) for both African American and Hispanic populations. The frequency of the major allele is presented (n/a = not available). Heterozygosity values were calculated based on the average allele frequency for each EST–SNP. The contig sequences and their corresponding GenBank accession numbers can be found in the NCBI SNP database under submitter handle ORCHID SNPS-981210-A.