Figure 4.
Expression pattern of genes across 30 human tissues, profiled by iAFLP. (A) An antisense primer for each of four genes was used to amplify six template pools (pools A through F) of human cDNAs adapted with iAFLP cassettes. The split peak represents partial terminal single nucleotide addition by Taq polymerase. (B) The pooling scheme to prepare six template pools with 30 human cDNAs. (C) Peak areas in each iAFLP profile were normalized relative to the peak area for reference cDNA mixture (30 mix). Note that the sum of normalized peak areas is close to the expected value of 30.
