Figure 2.
iAFLP profiles of standard template pools. Twenty-four standard template pools of different composition were made by adding one- (x2) to fourfold (x5) excess of each component to the even pool of six differentially adapted hepG2 cDNAs (LP40 through LP55). The GAPDH primer and 5-Fam T7(25) primer were used for amplification. Chromatographic pattern of PCR products (A) and the normalized area for each peak (B). Excess component (LP40 through LP55) and excess ratio (x2 through x5) are indicated.
