Construction of a Linkage Map of the Medaka (Oryzias latipes) and Mapping of the Da Mutant Locus Defective in Dorsoventral Patterning

Table 3.

Detection of Polymorphisms in the Known Genes by PCR Amplification

Gene  Acc. no Primer origin Primer pairs Fragment size (bp) Intron (bp) Polymorphism LG Homology   Location in human Location in zebrafish (LG)
graphic
  • Accession numbers are shown for the medaka nucleotide sequences used for designing primer pairs.

  • Primer pairs depicted in italics were first primer sets used for sequencing but not for segregation analysis.

  • Homology search was conducted against the nucleotide sequences of the genes from the HNI strain.

  • The locations of the human gene loci in the human genome were taken from the homepage (http://gdbwww.gdb.org/gdb/) or were referred to in Lyons et al. (1997).

  • The locations of the zebrafish gene loci in the zebrafish genome were referred to Postlethwait et al. (1998).

This Article

  1. Genome Res. 9: 1277-1287

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