Extensive Conservation of Sex Chromosome Organization Between Cat and Human Revealed by Parallel Radiation Hybrid Mapping
- 1Laboratory of Genomic Diversity, National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201 USA; 2Graduate Program in Biology, George Mason University, Fairfax, Virginia 22030 USA; 3Intramural Research Support Program, Science Applications International Corporation Frederick, National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201 USA
Abstract
A radiation hybrid (RH)-derived physical map of 25 markers on the feline X chromosome (including 19 Type I coding loci and 6 Type II microsatellite markers) was compared to homologous marker order on the human and mouse X chromosome maps. Complete conservation of synteny and marker order was observed between feline and human X chromosomes, whereas the same markers identified a minimum of seven rearranged syntenic segments between mouse and cat/human X chromosome marker order. Within the blocks, the feline, human, and mouse marker order was strongly conserved. Similarly, Y chromosome locus order was remarkably conserved between cat and human Y chromosomes, with only one marker (SMCY) position rearranged between the species. Tight linkage and a conserved gene order for a segment encoding three genes,DFFRY–DBY–UTY in human, mouse, and cat Y chromosomes, coupled with demonstrated deletion effects of these genes on reproductive impairment in both human and mouse, implicates the region as critical for Y-mediated sperm production.
[The sequence data described in this paper have been submitted to the GenBank data library under accession numbers AF197956–AF197962 andAF197964–AF197972.]
Footnotes
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↵4 Corresponding author.
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E-MAIL murphywi{at}mail.ncifcrf.gov; FAX (301) 846-6327.
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- Received June 16, 1999.
- Accepted August 23, 1999.
- Cold Spring Harbor Laboratory Press
