Error Analysis
| Tissue | Single consensus clusters | Total clusters (%) | Multi-consensus clusters | Total clusters (%) | Total only singletons | Total clusters (%) | Single consensus + 1 or more singletons | Total clusters (%) | 3′/5′ disagreement | Total clusters |
| Adipose | 173 | 96 | 5 | 3 | 3 | 2 | 0 | 0 | 23 | 13 |
| Brain | 19,933 | 87 | 1,850 | 8 | 296 | 1 | 769 | 4 | 2,552 | 11 |
| Cochlea | 689 | 97 | 13 | 2 | 4 | 0.5 | 4 | 0.5 | 18 | 3 |
| Connective | 4,098 | 88 | 316 | 7 | 93 | 2 | 140 | 3 | 358 | 8 |
| Digestive | 6,089 | 90 | 370 | 6 | 82 | 1 | 193 | 3 | 493 | 7 |
| Disease | 10,845 | 87 | 989 | 8 | 198 | 1 | 481 | 4 | 2,589 | 21 |
| Eye | 2,799 | 81 | 288 | 8 | 229 | 7 | 132 | 4 | 303 | 9 |
| Genomic | 14,924 | 91 | 792 | 5 | 177 | 1 | 421 | 3 | 2,550 | 16 |
| Gland | 10,843 | 88 | 820 | 7 | 237 | 0.2 | 408 | 4.8 | 1,096 | 9 |
| Heart | 7,341 | 88 | 622 | 7 | 104 | 1 | 274 | 4 | 699 | 8 |
| Hematolymph | 14,639 | 84 | 1,774 | 10 | 271 | 2 | 694 | 4 | 2,731 | 16 |
| Lung | 7,483 | 87 | 667 | 8 | 137 | 2 | 267 | 3 | 1,828 | 21 |
| Muscle | 1,084 | 92 | 64 | 5 | 12 | 1 | 23 | 2 | 67 | 6 |
| Olfactory | 238 | 96 | 7 | 2.8 | 2 | 0.8 | 1 | 0.4 | 4 | 2 |
| Other | 2,675 | 85 | 285 | 7 | 184 | 4 | 171 | 4 | 172 | 4 |
| Reproductive | 19,178 | 79 | 3,196 | 13 | 533 | 2 | 1,258 | 6 | 3,373 | 14 |
| Totals | 124,031 | 86 | 12,058 | 8 | 2,562 | 2 | 5,236 | 4 | 18,856 | 13 |
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CRAW analyzes cluster alignments generated by PHRAP or MSA_CONTIG and partitions consistent ESTs into subclusters based on agreement with other sequences. The ideal result is a single consensus cluster, accounting for 86% of the STACK output, while the remaining clusters may contain multiple sequence subclusters (resulting in a multiconsensus cluster), a primary consensus with one or more singleton sequences (data not shown), singleton ESTs according to the CRAW parameters. STACK clusters are generated by word identity counts and their read direction determined by majority vote of the annotations of constituent ESTs; clusters for which this vote is not unanimous (excluding abstentions) are noted in the right-most two columns.
