Table 2.
STACK Clustering and Alignment Results
| Tissue | Singletons | Total sequences (%) | Multisequence clusters | Sequence in MSC | Percent total sequences (clustering efficiency) | Small sequences | Total sequences (%) | Total sequences |
| Adipose | 1,693 | 71 | 181 | 572 | 24 | 111 | 5 | 2,376 |
| Brain | 42,245 | 24 | 22,848 | 130,573 | 73 | 4,458 | 3 | 177,719 |
| Cochlea | 1,973 | 46 | 710 | 2,213 | 51 | 118 | 3 | 4,304 |
| Connective | 12,652 | 31 | 4,646 | 26,210 | 64 | 876 | 2 | 40,753 |
| Digestive | 17,398 | 34 | 6,734 | 32,124 | 63 | 1,481 | 3 | 51,032 |
| Disease | 29,139 | 25 | 12,513 | 79,433 | 69 | 4,056 | 4 | 114,496 |
| Eye | 13,867 | 49 | 3,448 | 12,933 | 45 | 1,388 | 5 | 28,514 |
| Genomic | 38,481 | 38 | 16,314 | 72,066 | 71 | 4,457 | 4 | 101,986 |
| Gland | 25,836 | 23 | 12,307 | 62,176 | 55 | 1,672 | 1 | 112,346 |
| Heart | 20,782 | 30 | 8,341 | 45,795 | 66 | 217 | 0.3 | 69,830 |
| Hematolymph | 51,654 | 20 | 17,378 | 113,147 | 44 | 2,582 | 1 | 255,565 |
| Lung | 20,129 | 29 | 8,554 | 47,151 | 67 | 2,726 | 4 | 70,259 |
| Muscle | 4,534 | 28 | 1,183 | 8,792 | 54 | 1,037 | 6 | 16,237 |
| Olfactory | 1,478 | 56 | 248 | 830 | 32 | 283 | 11 | 2,600 |
| Other | 9,392 | 36 | 4,315 | 15,663 | 60 | 575 | 2 | 25,925 |
| Reproductive | 43,569 | 18 | 24,165 | 188,088 | 79 | 6,321 | 3 | 239,161 |
| Totals | 334,822 | 26 | 143,885 | 837,766 | 64 | 32,240 | 2 | 1,198,607 |
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The total sequences in each tissue set are partitioned by D2_CLUSTER into unique sequences (singletons) and clusters containing multiple related sequences [multi-seq clusters, (MSC)], whereas sequences of <50 bases are excluded from clustering (small sequences).
