Figure 7.
Overall strategy of sequencing error analysis. (a) Detection of a DNA region containing a putative sequencing error (BSERR54_ori), extraction of the two flanking regions (pm1, pm2primers) and PCR resequencing of the fragment (BSERR54_corr). (b) alignment of the BSERR54_ori and BSERR54_corr fragments, and (c) replacement of the erroneous fragment in the B. subtilis chromosome. Here, the correction shows that theycsA gene is actually longer than thought previously. Another frameshift error, circled in black, was additionally found by FSBlastX (BlastX hits are indicated by black rectangles).
