RNA in situ hybridizations to paraffin sections of mouse embryos, stages E14.5–17.5. (A-C) The global expression of G2in the nervous system is demonstrated. An even staining of spinal cord, dorsal root ganglia and brain with emphasis to the neopallial cortex can be observed as well as expression in the sympathetic trunk and the coeliac ganglion. Additionally, G2 transcripts are seen in the inner neuroblastic area of the retina, thymus, and the primordia of the teeth. (D-F) The anonymous cDNA clone 658218 detects an expression pattern in the nervous system that is very similar to that of G2. (G-I) The mRNA of the X component of the PDH complex is expressed mainly in skeletal muscle, diaphragm, and the heart, but also at lower levels in brain, spinal cord, and teeth primordia. (J-L) The GA17 mRNA is present in various tissues, mainly kidney (primitive glomeruli), lung, thymus, skeletal muscle, and teeth precursors, as well as in brain and spinal cord. Abbreviations: cg, coeliac ganglion; cm, condensing mesenchyme; dg, dorsal root ganglion; di, diaphragm; he, heart; hj, hip joint; il, iliac bone; ki, kidney; le, lens; li, liver; lm, primordium of lower molar teeth; lu, lung; nm, neck muscle; np, neopallial cortex; nr, neuroblastic layer of retina; gl, primitive glomeruli; ri, ribs; sc, spinal cord; sm, sternohyaloid muscle; st, sympathetic trunk; th, thymus gland; to, ex- and intrinsic muscles of the tongue; ui, primordium of upper incisor tooth; um, primordium of upper molar teeth.
