PAC contig covering 7.5 Mb of 11p13–14.1. 201 PAC clones are displayed, represented by horizontal bars with clone names (original well location) written above and insert sizes in kb below. PACs drawn as light grey horizontal bars between CAT and PAX6are from the PAC contig described previously in Niederführ et al. (1998). Square boxes at the ends of most clones allude to the SP6 (empty box) and the T7 ends (filled box) that have been used for hybridization analysis. Every PAC clone crossing a presumed vertical line through these boxes had shown a positive hybridization signal with the respective end probe and was thus proven to overlap. At thePAX6 locus several cosmid clones are shown that have been sequenced previously by the Sanger Centre (GenBank accession nos.Z83301, Z83306-83309, Z86001, and Z95332). The horizontal line on top of the contig represents the integrated map of 11p13–14.1 with known markers and NotI restriction sites that are indicated by tick marks. Genes are highlighted by shaded boxes and the newly integrated EST clones are written at a 45° angle. The positions ofRAG1/2 and TRAF6 as well as of the three EST clones 60587, 125727, and 41188, have been determined by hybridization to YAC clones located at the centromeric border of 11p13 (Gawin et al. 1995).
