A High-Resolution Physical Map of Human Chromosome 21p Using Yeast Artificial Chromosomes

Table 2.

PCR Primer Sequences and Chromosomal Assignments of Chromosome 21p Markers

Locus Clone Type Primer sequences Size (bp) Chromosomal assignment
D21S190 pVC1.21c STS CTGACAAGGAATTAGTAACC 133 13, 18, 21
TACACCTATTTGCCATTTGC
— ABM-C61 STR CCTAGCTTTCTGTCAGCAGT 450 13, 14, 15,21, 22
CAACATGATTCCACATAATGTA
— hmc01a06 EST TGGATGTCACTCTCATCCTTG 122 13, 22,21, Y
TCGAAGAAGAACATCCATGAG
— ABM-C62D STR CCACTGTTGTACAAATCAACTA 143 13, 15,21, Y
TCTAAGAATACCTGTAGGCATA
— Xba21 STS CAATCCCGTTTCCCACG 133 N.D.
CTTGTTTGTGATGTGTGCCC
D21Z4 pTRA-4 STS ATTTGGAGGCCTTTGTGGCTATGG 287 13, 14, 21
GGCCTCAAAACGCTCCAAGTATCC
D21Z3 pTRA-1 STS TGAGCCATTTGAGGCCTACTGTG 138 13, 14,21
TGTCTCCAAACTGCTCAATCAGAA
— ABM-C78 STR CCTCTTCTATGCCAGTCTTAA 109 9, 13, 14, 15, 20, 21, 22
CAGCTCTAAAGTAAGCTTGGA
D21S5 pPW235D STS CCAAAGGTTACTGCAGTCTC 123 13, 14,21
GGGGGTCATATAAAGGAAAC
D21S187 pVC10a STS TACATGCAAATGACCAAGAG 400 4, 9, 13, 14, 15, 21, 22
ATCAGCAATGCCTTCTAAGT
D21S1276 pLSB77 STS GGCCTGATGTCTGCCTTAGAT 108 1, 3, 9, 13, 14, 15, 20, 21, 22, Y
GCCATAGGTGAGCAACAGGA
D21S188 pVC1.12c STR TTCTGTGTCATCTGAACTGG 184 9, 13, 14, 15, 20, 21, 22, Y
ACGCACATTGAATACTGAGG
D21S191 pVC1.23c STS TGATTGGTATGACTTTGTTCCCAG 179 3, 4, 13, 14, 15, 21, 22
AGATGTCCACCCAGTGCTGC
D21S411 ABM-C74 STR GAACCATTATAAGTTGACCATC 196–206 3, 4, 13, 14, 15, 21, 22
ACATGTTAACATGCTATATCTGT
D21S1277 pAW14a STS AACTAAGGGCTCTAAAGCAT 300 3, 4, 9, 13, 14, 15, 21, 22, Y
AATGGGAGCACAAGTTCTAA
— 3C6 STS CAAGAGGGCACTGAACAG 59 1, 4, 13, 14, 15,21, 22, Y
GCACTCTTAGCTTTGCCA
D21S1278 pAW32a STS TCATACAGAGTATAACACCAGGAC 266 1, 10, 13, 14, 15, 20, 21, 22, Y
GTCTTATTGTGATAGGCTTGC
rDNA pCHB STS CGAGGTGACTCTCGGTTTGC 370 13, 14, 15, 21, 22
TTTCGCAAGCAGGCATTTG
rDNA pA2 EST TATGAACGCTTGGCCGC 204 N.D.
CGGTAACGCAGTGTCTAAGGC

  • (N.D.) Not determined.

This Article

  1. Genome Res. 9: 1059-1073

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