(A) Expression of potential a-specific genes in haploid and diploid cells. The expression of YJL169W (lanes1–5), YJL170C (lanes6–10), and PXA2 (lanes11–15) were assayed using RT–PCR. RNA prepared from α (lanes 1,6,11), a (lanes2,7,12), a induced with α factor (lanes 3,8,13), and a/α diploid (lanes 4,9,14) strains was used as the template with primers specific for each ORF. Genomic DNA was used as the template to serve as controls for the PCR reactions and the size of the expected fragments (lanes 5,10,15). (B) Expression of BAR1 (lanes1–3), MSG5 (lanes 4–6),YMR218C (lanes 7–9), and LAS21(lanes 10–12) in α (lanes1,4,7,10), a (lanes 2,5,8,11), and a/α diploid (lanes 3,6,9,12) strains by RT–PCR. (C) expression of genes by RT–PCR that show cell-type-specific expression by microarray assay. MFA1 (lanes1–3), SRA3 (lanes 4–6),PMP1 (lanes 7–9), and VPS13 (lanes10–12) in α (lanes 1,4,7,10), a (lanes2,5,8,11), and a/α diploid (lanes 3,6,9,12).
