Integration Sites per Chromosome and Location of pericentromeric and Most Telomeric Markers
| Chrom. | STSs (no.) | BACs (no.) | Genetic distance from most telomeric BACs to respective end of map (cM) | Pericent (cM) | |||||
| from ptel | from qtel | ||||||||
| 1 | 76 | 94 | 16.4 | D1S214 | 13.9 | D1S2693 | 4.6 (p12–q21.1) | ||
| 2 | 88 | 97 | 17.3 | D2S2164 | 37.5 | D2S2297 | 3.3 (p11.2–q11.2) | ||
| 3 | 55 | 64 | 25.4 | D3S3601 | 14.7 | D3S1314 | |||
| 4 | 46 | 51 | 22 | D4S1582 | 13.1 | D4S3074 | 6.7 (p12–q12) | ||
| 5 | 67 | 77 | 6.2 | D5S1980 | 0 | D5S2006 | 0.2 (p12–q11.2) | ||
| 6 | 57 | 58 | 27.1 | D6S259 | 19.3 | D6SS1585 | 6 (p11.2–q13) | ||
| 7 | 66 | 66 | 28 | D7S2495 | 12.7 | D7S798 | 5.1 (p11.2–q11.21) | ||
| 8 | 35 | 40 | 4.9 | D8S262 | 10.9 | D8S1837 | 5.4 (p11.2–q11.2) | ||
| 9 | 27 | 26 | 0.1 | D9S1779 | 6.3 | D9S1826 | 6.6 (p13–q13) | ||
| 10 | 48 | 51 | 3 | D10S1745 | 0 | D10S1700 | 3.3 (p11.2–q11.2) | ||
| 11 | 54 | 54 | 16.7 | D11S932 | 10.5 | D11S910 | 9.3 (p11.21–q12) | ||
| 12 | 48 | 55 | 12.6 | D12S314 | 3.4 | D12S343 | 13 (p11.2–q12) | ||
| 13 | 22 | 25 | 4.2 | D13S1236 | 12.3 | D13S1315 | |||
| 14 | 21 | 27 | 26.2 | D14S1071 | 3.9 | D14S1010 | |||
| 15 | 26 | 29 | 6.3 | D15S122 | 26.5 | D15S116 | |||
| 16 | 30 | 35 | 8.3 | D16S510 | 5.1 | D16S3063 | |||
| 17 | 25 | 25 | 0.6 | D17S926 | 41.1 | D17S807 | |||
| 18 | 19 | 20 | 5 | D18S1098 | 6.6 | D18S1115 | |||
| 19 | 10 | 10 | 24.7 | D19S873 | 5 | D19S404 | |||
| 20 | 22 | 23 | 2.9 | D20S103 | 13.1 | D20S853 | 1.5 (p11.1–q11.2) | ||
| 21 | 12 | 16 | 31.4 | D21S1901 | 1.3 | D21S1912 | |||
| 22 | 6 | 5 | 26.5 | D22S1147 | 11 | D22S1160 | |||
| X | 14 | 17 | 4.9 | AFMB290XG5 | 7.7 | DXS8069 | |||
| Y | 3 (shared w/ X) | 3 (shared w/ X) | |||||||
| Total | 873 | 965 | |||||||
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Genetic distances are given for marker-linked BACs mapping in bands closely flanking the centromeric regions. Meiotic interference in humans is suggested for chromosomes 1 and 9 but not 16, by comparison of the genetic distances between BAC–STS pairs that closely flank the centromere. For chromosome 1, only 4.6 cM is found betweenD1S2669 and D1S2343, mapping in 1p12 and 1q21, respectively, in comparison to 5 cM for two markers located within q21 (D1S2343 and D1S2714) and 40 cM between q21 and 125 (D2S2714 and D2S444), not likely matched by a 10-fold difference in DNA content. For chromosome 9, only 6.6 cM is found between D9S1791 and D9S273, mapping in 9p13 and 9q13, respectively, as compared with 22.1 cM from D9S273and D9S1790, mapping in q13 and 122.1, respectively; and 35 cM between D9S154 and D9S1826, located at the centromeric and telomeric borders of q34, again, not likely 5-fold the DNA content, although differential chromatin condensation must clearly contribute to the difference. Support for interference was not found for chromosomes 2, 5, 7, and 10, for which STS-BACs closely flanked the apparent centromeric regions and were associated with small genetic distance. For chromosome 2, a distance of only ∼3 cM separatesD2S2159 and D2S373/D2S2264, located in p11.2 and q11.2, respectively; see web site and Fig. 2). Definitive analyses over small distances will require physical and genetic maps such as that supporting interference across the chromosome 5 centromere (Puechberty 1999). Nonetheless, the current analyses support interference in the largest pericentromeric and heterochromatic blocks.
