Figure 1.
Schematic of the procedure for STS-based BAC mapping. (Step 1) Radiolabeled STS-probes are multiplexed and hybridized onto RPCI-11 segment 2 human male BAC library high-density filters. Liquid cultures of single colonies from positive clones are used to make dot blots. (Step 2) Individual probes are then hybridized onto the dot blots for verification and matching of clones to STS probes. (Steps 3 and 4) The mapped clones are then verified by two separate rounds of STS-content PCR, one round with liquid cultures of BAC clones (step 3) and another round with glycerol stocks of the mapped BAC clones (step 4).
