Table 1.

Interphase FISH Screening for Low-Alphoid Cell Lines

Chromosome		Chromosome-specific α-satellite probes		No. of cell lines		No. of low-alphoid centromeres
Chromosome		Chromosome-specific α-satellite probes		phase I	phase II	total	phase I	phase II	total	prevalence (%)
1	pZ5.1	—	328	328	—	—	—	—
2	pX2	78	290	368	—	—	—	—
3	pAE0.68	62	301	363	—	—	—	—
4	p4n1/4	74	243	317	—	—	—	—
5	pZ5.1	—	328	328	—	—	—	—
6	pEDZ6	76	245	321	—	—	—	—
7	pZ7.5	77	319	396	—	—	—	—
8	pZ8.4	76	227	303	—	—	—	—
9	pMR9A	65	320	385	—	—	—	—
10	pZ10-2.3	70	266	336	—	—	—	—
11	Oncor D11Z1	76	312	388	—	—	—	—
12	pB12	78	306	384	—	—	—	—
13	αRI	53	366	419	—	1	1	0.12
14	αXT	73	321	394	—	—	—	—
15	pTRA20	58	328	386	—	—	—	—
16	pZ16A-2	67	241	308	—	—	—	—
17	TR17	78	357	435	—	1	1	0.11
18	L1.84	69	239	308	—	—	—	—
19	pZ5.1	—	328	328	—	—	—	—
20	pZ20	77	268	345	—	—	—	—
21	αRI	53	366	419	6	25	31	3.70
22	αXT	73	321	394	—	—	—	—
XY	XY α-mix	67	294	361	—	—	—	—

↵Prevalence is expressed in terms of the total number of chromosomes.
↵For chromosomes 1, 5 and 19, screening for low-alphoid centromeres was done on metaphase chromosomes.
↵This mix contains the X- and Y-specific probes, pLAX and pLAY5.5, respectively.

Extreme Reduction of Chromosome-Specific α-Satellite Array Is Unusually Common in Human Chromosome 21