Figure 5.
Q-FISH analysis of the centromeric αRI domain and the 876D4 genomic region at 21q22.1–22.2 in the human chromosome 21 somatic cell hybrid WAVR-4d-F9-4a. (A) The 876D4 signals (solid arrows) on the long arms of sister chromatids of chromosome 21 can easily be distinguished from the centromeric α-satellite signals (open arrow). (B) Each point represents the fluorescence intensities of αRI and 876D4 probe on the human chromosome 21 in different metaphase spreads on the same slide. Data are fitted to a straight line by linear regression. (a.f.u.) Arbitrary fluorescence units.
