Error Checking and Graphical Representation of Multiple–Complete–Digest (MCD) Restriction-Fragment Maps

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Figure 3.
Figure 3.

(Top) The EcoRI portion of the MCD map; (bottom) line segments showing both the minimum and maximum clone lengths allowable for this clone according to the EcoRI data. Below these two possible clone lengths, the actual clone length as an average of the clone’s lengths from all three enzyme domains is shown. This is obviously too large a clone to fit between the flankingEcoRI restriction sites. Each variable is located directly above the restriction site with which it corresponds, omitting those that are too close together to be distinguished from each other. g1980a113’s left clone end has been positioned betweenx 1 and x 2, and its right end lies between x 10 and x 11 that bound the 568-bp fragment. The inequalities representing this physical inconsistency are explained in the text in terms of these variables.

This Article

  1. Genome Res. 9: 79-90

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