Error Checking and Graphical Representation of Multiple–Complete–Digest (MCD) Restriction-Fragment Maps

Our graphical representation for a single-enzyme restriction map consists of a horizontal line depicting the mapped target DNA with individual restriction sites indicated by vertical line segments as shown here. Once a choice for zero (O) is made, the distance from the left end of the map to any particular group boundary (or restriction site) can be determined and is represented by the variablesx_i . The distances from zero to the left and right ends of each clone is represented by the variablesl_m and r_m , respectively. In the formalism presented for the system of constraints on these variables, the length of each clone, denoted by L_m , as well as the length of each group of restriction fragments,G_k , are determined from the data available in each MCD map.