RT–PCR analysis of the expression of Cdc2L transcripts originating from the two different CpG promoter regions in various human tissues and cell lines. (A) RT–PCR analysis of CpG1 and CpG2 (see Fig. 1) transcripts expressed by Cdc2L1 andCdc2L2. The various tissues and cell lines are indicated above each lane, whereas five different sized products (see text for explanation) are indicated on the right of each panel. (B) RT–PCR analysis of the expression of CpG1 transcripts corresponding to the Cdc2L1 gene, using the same set of tissue and cell line RNAs. The arrows at right indicate that these CpG1 mRNAs are expressed in a few, but not all, of the sources examined. Decreased resolution of the two RT–PCR products (bottom) is caused by the short run of the agarose gel used to analyze the RNAs from the various cell lines.
