PCR–LR analysis of Cdc2L1 and Cdc2L2 mRNAs expressed by various cell lines. An analysis of the expression of Cdc2L1and Cdc2L2 using a unique EcoRI restriction site found in exon 17 of Cdc2L2 (bottom) was performed by RT–PCR. The undigested (−) and EcoRI-digested (+) RT–PCR products were separated by agarose gel electrophoresis, transferred to a membrane, and hybridized with the PITSLRE cDNA. Whereas most cell lines express both Cdc2L1 andCdc2L2 transcripts, Daudi and U937 cells appear to express primarily Cdc2L1 mRNA, which lacks the diagnosticEcoRI site. (Bottom) A schematic of Cdc2Lstructure relative to the location of the PCR primers used in this analysis. The expected sizes of the undigested (539 bp) and digested (359 and 180 bp) products is also indicated.
