Organization of the pMCH gene, illustrating the region of sequence overlap for the pMCH gene on 12q and the human–variant pMCHL genes on 5p/5q, and showing the sites of sequence difference between the 5p and 5q human–variant loci. The 5p sequence is presented above the 5q sequence, with the points of difference in uppercase letters. Also shown are the primers used forpMCHL-specific PCR amplification (P2 and P7). The region with slanted lines indicates the coding region for the peptide—neuropeptide glycine–glutamic acid (NGE). The region with horizontal lines indicates the coding region for the peptide—neuropeptide glutamic acid–isoleucine (NEI). The regions with open circles indicate the coding region for MCH. It is not known whether the exon/intron structure is conserved in the region of homology between pMCHand pMCHL genes.
